PRODUCTION OF HIGHLY PURIFIED BOVINE TRANSFERRIN Group 5B Arjun Goel, Connie Marcelli, Lianne Kark, Sara Dang, Vivien Tai Faculty Advisor Prof C.A. Mims

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PRODUCTION OF HIGHLY PURIFIED BOVINE
TRANSFERRINGroup 5BArjun Goel, Connie
Marcelli, Lianne Kark, Sara Dang, Vivien
TaiFaculty Advisor Prof C.A. Mims
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Timetable
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Agenda

• Mass Balances Waste Treatment
• Key Unit Discussion
• Unit Specifications
• Current Challenges

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Process Overview
Crude Paste
Filtration
Chromatography
Dry Transferrin Powder
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Agenda

• Mass Balances Waste Treatment
• Key Unit Discussion
• Unit Specifications
• Current Challenges

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Mass Balance Feeds
Starting Paste 18 kg
Distilled Water 160 L
FeCl3, NaHCO3, Na-Citrate for iron Binding
Celite 6 kg
D.I. Water 571 L
Process
EDTA for Iron Chelating
Diafiltration Buffers
Wash for Column
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Mass Balance Products
Solids
Liquids
Immunoglobins for recovery
Trace Proteins
Water 740 L
Inorganics FeCl3, NaHCO3, Na-Citrate, EDTA, NaOH
Process
Ethanol 4.5 kg
Celite 6 kg
Purified Apotransferrin 350g / Batch
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Waste Treatment--Liquids

• Option 1

• Option 2

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Waste Treatment Solids

• Reverse Osmosis cartridges
• Filter press cakes
• Disposal via contractor
• Mainly salts and celite trapped in filters

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Agenda

• Mass Balances Waste Treatment
• Key Unit Discussion
• Unit Specifications
• Current Challenges

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Key Unit R-101
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Key Unit R-101

• Stirred Tank
• Cylindrical
• Conical Bottom
• Tank Material SS316
• In Cold Room 0-5 oC

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Key Unit R-101

• Volume of solution in tank 400 L
• Unit Selected
• Capacity 757 L ( 200 Gal )
• Depth 1.46 m
• Diameter 0.91m

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Key Unit Accessories

• Level Indicator
• pH Monitor
• Temperature Indicator
• SS316 piping
• Valve
• Air Diaphragm Pump
• Mixer

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Agenda

• Mass Balances
• Waste Treatment
• Key Unit Discussion
• Unit Specifications
• Current Challenges

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Filter Press FP-101

• 12 in diameter
• Stainless Steel plates and frames
• Cellulose Pads
• Four 1 sludge frames
• 40 psi
• Cost 6 703

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Filter Press FP-101

• 3 Applications
• 4 Plates removes 12 000 g paste _at_ 19 L/min
• 2) 2 Plates removes 1 320 g denatured protein _at_
  9 L/min
• 3) 2 Plates collects 3 600 g precipitated
  protein _at_ 9 L/min

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PrefiltrationSF-101

• Before ultrafiltration
• Millipore low protein binding cartridge filter
• Filtration Area 0.7m2
• 80 psi
• Entrains particles gt 0.5 mm
• US208 for 2

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Ultrafiltration
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Spiral Wound FilterUF-101

• Concentration 450 L to 90 L
• Polysulfone
• Flow 100 L/hr
• Flux 22 L/m2 hr
• Pressure 60 PSI
• Cost 8 212

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Spiral Wound FilterUF-102

• Concentrate from 30L to 15L
• Regenerated cellulose Membrane
• Pressure 60 PSI
• Flow 10L/hr
• Flux 11 L/m2hr
• Cost 2 154

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Hollow FiberUF-201

• Concentrate from 12 L to 6 L
• Polysulfone
• 25 PSI
• 6 L/hr
• 30 L/ m2hr
• Peristaltic Pump

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DEAE Column
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DEAE Sepharose Fast Flow Column C-201

• Automated
• Includes The Column
• The Bioprocess Skid
• The computer
• DEAE Resin
• Total Cost CND211, 000

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Water Distillation ColumnT-204

• Rate 190 L/24 hrs
• Storage capacity 280 L
• Size
• 2m x 1m x 0.6m
• Cost US 3 945

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Deionized Water UnitI-101

• Flow range
• 15 23 L/min
• Size
• 1.5m x 0.6m x 0.4m
• Cost
• US 4 142

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LyophilizerL-101

• Similar unit used in plant
• Capacity 8-12 L
• 6 L of water to condense per batch

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Future Directions

• Key unit development
• PID
• Design equations
• Control Philosophy
• Economic Evaluation
• Plant Layout
• Appendices