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Nematode Extraction Methods: Foliar Extraction Techniques

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Sampling and extracting for nematodes serves two purposes ... Joey Orajay, Entomology and Nematology Department, University of Florida, Gainesville, FL ... – PowerPoint PPT presentation

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Title: Nematode Extraction Methods: Foliar Extraction Techniques


1
Nematode Extraction MethodsFoliar Extraction
Techniques
  • Kavitha Govindasamy
  • Jimmy R.Rich
  • Maria L .Mendes

2
A Plant-Parasitic Nematode
3
Sampling for Nematodes
  • Sampling and extracting for nematodes serves two
    purposes
  • Diagnosis a current problem
  • Predict a future problem

4
Objectives of sampling and extraction
  • Identify the nematode
  • Population estimation
  • Diagnose a nematode disease
  • Make management decisions
  • Regulatory purposes
  • Research and surveys

5
Some Common Nematode Extraction Methods
  • Foliar samples
  • Punching and incubating
  • Scissors technique
  • Soil samples
  • Baermann funnel
  • Modified Baermann funnel
  • Root Samples
  • Jar incubation and sieving
  • Blending and sieving

6
Foliar Extraction TechniquesUsing a punch or
scissors
  • Principle
  • Nematodes migrate out of the leaves when the
  • lesions are soaked in water

7
Materials Required
Hole punch or scissors Petri dish or other
container
8
Cut leaf tissue and place in water
9
Punch the lesions and place tissue disks in water
10
Take samples near healthy tissue
11
Incubate 24 hours and observe under microscope
12
Acknowledgments
  • Janete Brito, Department of Plant Industry,
    Gainesville, Florida
  • Frank Woods, Nematode Assay Lab, University of
    Florida, Gainesville, Florida
  • Joey Orajay, Entomology and Nematology
    Department, University of Florida, Gainesville,
    FL

13
For additional information or comments, please
contact
  • Kavitha_nem_at_yahoo.com
  • jimmyr_at_ufl.edu
  • mlmendes_at_ufl.edu
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