Incidence and Survival of Campylobacter

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Incidence and Survival of Campylobacter and
Salmonella in Animal Faeces, Surface Waters and
Groundwaters
Lester Sinton, Elaine Moriarty, Louise Weaver
Specialist Science Solutions
Manaaki Tangata Taiao Hoki protecting people and
their environment through science
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ESR Research Programmes
Potentially zoonotic bacteria and
protozoans 1. Animal faecal microbe reservoir
models 2. Survival of Campylobacter and
Salmonella in surface waters 3. Survival and
transport of Campylobacter in groundwaters 4.
Survival and transport of Campylobacter in the
vadoze zone
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Faecal Microbe Reservoir Models
Tool to estimate reservoir sizes for cattle,
sheep and waterfowl Model Inputs (cattle and
sheep) - Animal numbers - Microbial counts in
fresh faeces - Survival in deposited
faeces - Leaching rates from deposited faeces
Models intended to be stand-alone, and as
inputs to farm and catchment scale models
developed by AgResearch, NIWA, and Landcare
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Faecal Microbe Reservoir Models
Completed - National survey of dairy cattle
(Elaine Moriarty) - Microbial survival in cow
pats (Lester Sinton) - Microbial survival in
sheep faeces (Elaine Moriarty) Underway - Surve
y of sheep faeces (Elaine Moriarty) - Survey of
waterfowl faeces (Elaine Moriarty) Proposed - L
eaching rates from deposited faeces (Louise
Weaver) - Survival in wildfowl faeces (Elaine
Moriarty)??
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National Survey of Dairy Cattle
Sampled 10, separate, fresh, cow pats - Four
dairy farms - Waikato, Manawatu, Canterbury,
Southland - Four times a year - 155
pats Bacteria - E. coli, enterococci,
Campylobacter, Salmonella, STEC Protozoans - Gi
ardia, Cryptosporidium
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National Survey Results
No Salmonella detected Two samples positive
for STEC 4.5 positive for Giardia - Low
counts (1 17 g-1) - Autumn only - Waikato and
Manawatu 5.2 positive for Cryptosporidium - L
ow counts (1 25 g-1) - Autumn only - Waikato
and Manawatu
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Campylobacter Results
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Campylobacter Results
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Survival in Cattle Faeces
Placed 10, replicate, composite, pats on
pasture - Each of 4 seasons - Half the pats
used to sample E. coli, faecal streptococci and
enterococci - Seeded half the pats with
Campylobacter and Salmonella - Sampled until
disintegration (usually around 5 months)
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Survival in Cattle Faeces
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Survival in Cattle Faeces
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Survival in Cattle Faeces
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Survival in Cattle Faeces
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Survival in Cattle Faeces
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Survival in Cattle Faeces
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Survival in Surface Waters
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Survival in Surface Waters
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Survival in Groundwater
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Transport in Groundwater
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Transport in Groundwater
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Transport in the Vadoze Zone
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Acknowledgments
Margaret Mackenzie, Naveena Karki, Mark
Fintoft NIWA, AgResearch, Lincoln
Ventures Funded from the PGSF, administered by
FRST