GENOMIC IMPRINTING AND EPIGENETICS IN GENES OF ECONOMICAL INTEREST IN CATTLE

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GENOMIC IMPRINTING AND EPIGENETICS IN GENES OF
ECONOMICAL INTEREST IN CATTLE

• GENOMICS WORKSHOP ESALQ / USP
• Piracicaba, December 17th 2007

Simone Cristina Méo Niciura simone_at_cppse.embrapa.b
r
Pecuária Sudeste
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RÉSUMÉ

• Simone Cristina Méo Niciura
• Graduation (1999) Veterinary Medicine FCAV /
  UNESP Jaboticabal, SP
• MS (2002) / PhD (2005) Veterinary Medicine
  Animal Reproduction FCAV / UNESP Jaboticabal,
  SP
• Affiliation (March 2007) Embrapa
  Cattle-Southeast São Carlos, SP

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PREVIOUSWORKS
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PARTHENOGENETIC ACTIVATION

• Activation of bovine oocytes with ionomycin
  followed by 6-DMAP (ID) or by strontium chloride
  (IS)
• Recovery of viable parthenogenetic bovine embryos
  (ID) on Day 35-36 of pregnancy

Méo-Niciura, 2005
Pecuária Sudeste
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EXPRESSION OF IMPRINTED GENES IN FETUS AND
PLACENTA OF PARTHENOGETIC, IN VITRO AND IN VIVO
PRODUCED BOVINE EMBRYOS
Méo-Niciura, 2005
Pecuária Sudeste
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EXPRESSION OF IMPRINTED GENES AND DNMTs IN
PARTHENOGETIC, SCNT, IN VITRO AND IN VIVO
PRODUCED BOVINE EMBRYOS
Perecin, 2007
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EXPRESSION OF IMPRINTED GENES IN PLACENTA AFTER
DELIVERY OF SCNT (MALE vs. FEMALE) AND IN VIVO
PRODUCED BOVINE EMBRYOS
Yamazaki, 2006
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FUTURE INTERESTS
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DIFFERENTIAL EXPRESSION

• Differential Allelic Expression allelic
  variation in gene expression where alleles of
  non-imprinted genes are not expressed equally at
  the mRNA level (Khatib, 2007)
• Parent-of-origin effects on gene expression not
  caused by genomic imprinting
• Drosophila melanogaster (Wittkopp et al., 2006)
  and prairie voles (Microtus ochrogaster Kramer
  et al., 2006)
• Bovine at DGAT1 gene with effects on milk
  production traits (Kuehn et al., 2007)

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DIFFERENTIAL EXPRESSION

• Genes of economical importance
• It seems possible that knowledge about the
  influence of pathway (paternal or maternal) used
  by an allele to enter the next generation can be
  adopted in some selection programs (Ruvinsky,
  1999)

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IDEAS

• Genotyping of SNP in candidate genes for meat
  tenderness, subcutaneous fat deposition and
  marbling
• Evaluation of tissue-specific expression,
  differential allele expression, and
  parent-of-origin effects on expression of genes
  of economic importance in beef cattle
• Evaluation of epigenetic mechanisms (DNA
  methylation, histone modifications) controlling
  gene expression
• Evaluation of cellular differentiation in vitro
  after allele-specific transient silencing using
  RNAi

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IDEAS
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IDEAS
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THANK YOU!
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