Posttranscriptional regulation of Egr1 in the gonadotrope is influenced by CSDA Theodore R' Chauvin

1
Post-transcriptional regulation of Egr1 in the
gonadotrope is influenced by CSDA Theodore R.
Chauvin John H. NilsonSchool of Molecular
Biosciences, Washington State University,
Pullman, WA, USA Center for Reproductive
Biology, Pullman WA, USA
The effect of GnRH on Egr1 mRNA half-life
RNA was isolated and Northern blot analysis
performed

• The 3 UTR of Egr1 was inserted into a luciferase
  reporter vector and used in transient
  transfection assays in order to identify regions
  of the UTR which may be important for mRNA
  stability

Conclusions

• GnRH appears to have no effect on the half life
  of Egr1 mRNA
• The 3 UTR of Egr1 enhances luciferase activity
  in transfected LbT2 cells
• The 3 Csd sequence in the proximal region of
  the Egr1 UTR appears to modulate luciferase
  activity in transfected LbT2 cells
• Reduction of CSDA by siRNA can also modulate
  activity of luciferase in transfected LbT2 cells
• Reduction of CSDA by siRNA reduced GnRH
  stimulation of both a transfected LHB-luciferase
  construct and endogenous Lhb