Detection of Ruminant MBM Prohibited Protein

1
Detection of Ruminant MBM Prohibited Protein

• Development of an assay to address the needs of
  the feed industry.
• Ensure consumer confidence in FDA regulatory
  compliance.

2
U.S. Livestock Industries Impacted

• Beef cow-calf
• Beef feedlot
• Dairy
• Rendering Facilities
• Blenders and Formulators
• Feed manufacturing

3
Industry Developments

• 1986 Confirmation of BSE in EU.
• 1988 EU bans ruminant proteins.
• 1996 National livestock organizations
  adopt voluntary ban on use of
  ruminant-derived proteins.
• 1997 FDA bans ruminant-to-ruminant
  feeding of proteins.
• 2001 Texas feedlot/feed manufacturer
  incident. FDA Inspection
• 2001 Proposed Expansion of FDA feed ban.

4
U.S. Rendering Industry

• 35 million head slaughtered each year.
• Death loss of 1-2 annually.
• 260 rendering firms in U.S.
• Yields 3 million tons/year of rendered beef
  products.

Source Kansas State University
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U.S. Rendering IndustryNon Ruminant By-Product

• Yields 1.5 mil tons/year Poultry meal.
• Yields 0.6 mil tons/year Feather meal.
• Yields 0.8 mil tons/year Porcine meal.
• Yields 0.1 mil tons/year Blood meal.

Source Sparks study for NRA, June 2001
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U.S. Rendered Product Utilization

• Feed Industry 3 mil tons.
• Pet Food Industry 1.5 mil tons.

Source Sparks study for NRA, June 2001
7
Current Predicament

• Feeding of rendered products is integral to
  economic viability of livestock industries.
• Utilization of Ruminant Byproduct is regulated.
• Currently NO rapid detection system in use to
  verify compliance with FDA Prohibited Material
  regulation.

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Ruminant MBM
Detection at 5 Meat and Bone Meal Detection
at 1 Finished Feed Feed Supplements Feed
Ingredients Format Dipstick Immunoassay Simple
Sample Prep Reliable Results
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AgriScreen Ruminant MBMStatistics
Test for MBM in Animal Meals

• Sensitivity 97.6
• Specificity 99.5
• 170 Positive, 187 Negative
• n 357

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AgriScreen Ruminant FEEDStatistics
Test for MBM in Animal Feeds

• Sensitivity 98.7
• Specificity 99.5
• 308 Positive, 218 Negative
• n 526
• Submitted for AOAC Validation

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Step 1 - Weigh Sample
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Step 2 - Extraction Additive
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Step 3 - Extraction Solution
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Step 4 - Heat Sample
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Step 5 - Transfer Sample
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Step 6 - Insert Device
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Step 7 - Interpret Result
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Ruminant Feed Sensitivity
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AgriScreen Ruminant MBM Specificity
1 gram / 100 mL 1 - Lamb 2 - Deer 3 -
Elk 4 - Beef 5 - Goat 6 - Chicken 7 -
Turkey 8 - Pork 9 - Fish 10 - Horse 11 - Sheep
Control
Test
1 2 3 4 5 6 7 8 9 10 11
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Heat Treatment for Bovine MBM

• 1. Agri-Screen for Ruminant in MBM
• Strip Test
• MBM (50 Beef/Pork)
• produced at 5 different defined t
• by EU JRC (Ispra-Italy)

The signal decreases with the t elevation but
the test is positive with all samples tested.
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Ruminant MBM Byproduct Screening
1 - Bovine MBM 2 - Bovine MBM (110) 3 - Ovine
MBM 4 - Ovine MBM (110) 5 - Porcine MBM 6 - Fish
Meal 7 - Blood Meal (porcine) 8 - Blood Meal
(bovine) 9 - Poultry Meal 10 - Feather Meal 11 -
Poultry Meal (low ash)
1 2 3 4 5 6 7 8 9 10 11
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Ruminant MBM Test
Samples 1- Pork MBM 2 - Pork 5 Beef MBM 3 -
Feather Meal 4 - Feather 5 Beef 5 - Turkey
Meal 6 - Turkey 5 Beef 7 - Chicken Meal 8 -
Chicken 5 Beef 9 - Fish Meal 10 - Fish 5
beef
1 2 3 4 5 6 7 8 9 10
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AgriScreen Method Comparison
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Ruminant MBM Commodities
Evaluated Commodities 53 Animal Meals 57 Feed
Ingredients 189 Finished Feeds
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AgriScreen Feed Test Specificity
1 gram / 100 mL 1 - Cow 2 - Sheep 3 - Goat 4 -
Deer 5 - Elk 6 - Horse 7 - Pig 8 - Rabbit 9 -
Turkey 10- Chicken
1 2 3 4 5 6 7 8 9 10
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AgriScreen Feed Test Sensitivity
Feed Beef MBM 1 - Neg Feed 2 - 0.5 3 - 1
4 - 2 5 - 5 Sheep MBM 6 - Neg Feed 7
- 0.5 8 - 1 9 - 2 10 - 5
BEEF SHEEP
1 2 3 4 5 6 7 8
9 10
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Sensitivity
Samples of Feed with 0 and 0.5 of bovine
MBM produced by the Australian Government
Analytical Laboratory Detection at 0.5 level
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Domestic MBM Detection
MBM at 1 1 - Neg. Feed 2 - MBM 3 - MBM 4 -
Beef MBM 5 - Lamb MBM 6 - Beef MBM 7 - MBM 8 -
MBM 9 - Lamb MBM 10 - MBM
1 2 3 4
5 6 7 8
9 10
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International MBM
Samples of Feed adulterated with American MBM
(0.5 ) Australian MBM (0.5 ) European MBM (0.5
and 1 ) Detection at 0.5 level
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Ruminant Feed Commodities

• Animal Feeds
• Animal Feed Dry Supplements
• Animal Feed Ingredients
• - Vegetable Proteins
• - Mineral source
• - Fats and Oils
• - Milk Replacers

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Ruminant Detection LOD
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Current Predicament

• Feeding of rendered products is integral to
  economic viability of livestock industries.
• Utilization of Ruminant Byproduct is regulated.
• Currently a rapid detection system to verify
  compliance with Feed Ban regulation does exist.
• Inexpensive screening of samples is possible.