SERUM CYTOKINE PATTERNS IN CELIAC DISEASE - PowerPoint PPT Presentation

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SERUM CYTOKINE PATTERNS IN CELIAC DISEASE

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B) Gluten Free Diet (GFD) (43) C) Refractory disease (6) D) Controls (16) ... Patients on therapy (gluten-free-diet) had fewer pro-inflammatory cytokines with ... – PowerPoint PPT presentation

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Title: SERUM CYTOKINE PATTERNS IN CELIAC DISEASE


1
SERUM CYTOKINE PATTERNS IN CELIAC DISEASE
  • Shabab Naqvi Ibrahim Ibrahim
  • Mentor Dr. Sanil Manavalan
  • Columbia University, College of Physicians and
    Surgeons

2
  • Celiac disease
  • a digestive disease that damages the small
    intestine and interferes with absorption of
    nutrients from food
  • it is a genetic disorder
  • some of the symptoms of celiac disease are
    diarrhea, weight loss, malnutrition, etc
  • when people with celiac disease eat foods
    containing gluten, their immune system responds
    by damaging the small intestine
  • Diagnosis is by intestinal biopsy histological
    grading and presence of serum auto- antibodies.

3
Key Terms
  • Cytokines soluble peptides or glycoproteins
    that regulate immune, inflammatory responses
    and other biologic processes.
  • Interleukin (IL) cytokines that only affect
    leukocytes.
  • Lymphocyte A type of leukocyte (white blood
    cell) of the immune system.
  • T-Regulatory Cell slows down and stops immune
    response
  • T-Helper Cell (Th cell) has antigen-receptors,
    releases cytokines
  • Natural Killer Cell kills macrophage and bad
    stuff
  • Cytotoxic T-Cell kills cells that produce
    foreign antigens such as cells infected by
    viruses
  • B-Cell creates antibodies.
  • Antigen invading microbe
  • Antibody protein that fights bacteria and
    viruses.

4
The Gut Lumen
5
(No Transcript)
6
GUT LUMEN
GUT LUMEN
Gliadin
Enterocytes
LAMINA PROPRIA AND INTRA EPITHILIAL SPACE
7
AIMS
Define the serum cytokine profile in patients
with Celiac Disease
A) Active Disease B) Gluten Free
Diet (GFD) C) Refractory disease (RD)
D) Normals/Controls
8
METHODOLOGY
Serum Samples from A) Active Disease (25) B)
Gluten Free Diet (GFD) (43) C) Refractory
disease (6) D) Controls
(16) Measured using a Cytokine Bio-chip Array
(RANDOX laboratories Ltd) Mann-Whitney Rank
test and Student T-test to determine differences
in the means of individual groups of patients
Cytokines Measured
Th-1 Derived IL-2 IFN-?
Th-2 Derived IL-4 IL-6 IL-10
APC Derived IL-8, IL-1a, IL-1b, TNF-a
9
RESULTS
P 0.01
P 0.006
P 0.06
IL-4 (pg/ml)
P lt0.005
P 0.04
P 0.004
IFN-g (pg/ml)
10
ACTIVE CD VS. NORMALS
Active CD n 25 Controls n 16
11
GFD vs. Normals
APC Derived
Th-2 Derived
Th-1 Derived
GFD n 43 Controls n16
12
SUMMARY
  • Patients with Celiac Disease (AD, GFD or RD) have
    significantly higher levels of pro-inflammatory,
    TH1( (IFN-?) and TH2 (IL-4, IL-6, IL-8) cytokines
    when compared to normal controls.
  • Patients on therapy (gluten-free-diet) had fewer
    pro-inflammatory cytokines with elevated levels
    than Active disease patients

13
CONCLUSIONS
  • A better understanding of cytokine abnormalities
    may provide insight
  • into
  • -Systemic symptoms
  • -Disease pathogenesis
  • -Better monitoring of disease
    activity
  • -New therapeutic and prognostic
    modalities

14
THANK YOU
  • To Dr. Sanil Manavalan
  • Dr. Jamal Naiyer
  • Dr. Sat Bhattacharya
  • Harlem Children Society
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