Phylogenetic analysis of flatfish species (Teleostei, Pleuronectiformes) based on cytochrome oxidase 1 (Co-1) and cytochrome b (Cyt-b) genes

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Phylogenetic analysis of flatfish species
(Teleostei, Pleuronectiformes) based on
cytochrome oxidase 1 (Co-1) and cytochrome b
(Cyt-b) genes

• Sharina S.N., Kartavtsev Y.P.

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INTRODUCTION

• The phylogenetic relationships within the order
  are still problematic
• Fish mitochondrial DNA is now widely used with
  phylogenetic purposes
• Most popular in phylogenetics are sequences of
  cytochrome b (Cyt-b) and cytochrome oxidase 1
  (C?-1) genes, which used for taxa comparison at
  the species-family level.

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Aim Molecular phylogenetic investigation of
relationships among order Pleuronectiformes

• To make reconstruction the phylogenetic
  relationships based on Co-1 and Cyt-b gene
  sequencing among some flatfish species were made.
• To confirm or disprove the monophyly of the
  flatfish family Pleuronectidae and genus
  Pleuronectes several tree types were built.

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Materials and methodsSamples of the collection
Kievka Bay
Vostok Bay
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• (Kartavtsev et al., 2007)
• Flatfish-cytb-F ATGGCCAACCTCCGTAAATCCCACCCCCTTC
• Flatfish-cytb-R CTGGGGCTCTGGACGCTGAGCTACTAGTGC
• (1441 bp)

20 spesies of Pleuronectiformes for Co-1 gene 47
species of Pleuronectiformes for Cyt-b gene 2
species of Gadiformes as outgroup

• Flatfish-1-F TCTTTAGATTTGCAATCTAACATGTA
• Flatfish-1-R GTCAGTAGCATTGTAATTCCTGCGGC
• Flatfish-2-F TGGGCCCATCACATATTTACAGTCGG
• Flatfish-2-R CAGAGCGGTTATGTGGTTGGCTTGAA
• (1500 bp)

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Rooted consensus (50) MP-tree showing
phylogenetic interrelationships on the basis of
Co-1 sequence data. In the nodes bootstrap
support (n1000) for MP and NJ (MP/NJ) are shown.
The tree was rooted with the sequences of two
out-group species Gadiformes.
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Rooted consensus (50) BA-tree showing
phylogenetic interrelationships on the basis of
Cyt-b sequence data. In the nodes bootstrap
support (n1000) for NJ and MP and repetition
frequencies for n106 simulated generations for
BA (BA/MP/NJ) are shown. The tree was built based
on the TrNIG model and was rooted with the
sequences of two out-group species Gadiformes.
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Hippoglossinae
Hippoglossoidinae
Pleuronectinae
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Conclusions

• Family Pleuronectidae with high confidence may be
  considered as monophyletic.
• According the last systematical revision, we have
  sampled in the genus Pleuronectes only one
  species P. pinnifasciatus. Other species, placed
  in different genera Pseudopleuronectes,
  Liopsetta, and Lepidopsetta.
• The diagnostics of species (barcoding) based on
  Co-1 and Cyt- b gene sequences is highly
  effective because of low intraspecies and high
  interspecies variability of this markers.

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THANKS FOR ATTENTION!