Microscopes

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Microscopes

• Drawing Rules, Magnification Field of View

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Microscope Drawing Rules

• Use a pencil
• Neat, ruled lines that do not overlap
• All labels to the right
• No arrows on lines
• Each diagram at least half a page

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Microscope Drawing Rules

• Stipple in darker areas (no shading)
• Magnification should be written at the bottom of
  the diagram
• Descriptive Title Should be UNDERNEATH the
  drawing (Figure 1 ___________)

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Field of View (FOV)
Field of View the view you actually see when
you look through a microscope
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Microscope Calculations
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Field of View (FOV)
Field of View the view you actually see when
you look through a microscope
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Low Field of View (Low FOV)

• Low FOV
• measured with a ruler on the microscope

• Count the spaces you see
• Count the halves as well
• Here we see 5.5 spaces
• So Low FOV 5.5 mm

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Medium High Field of View (FOV)

• Medium FOV
• low FOV X _MagnificationLOW_
• MagnificationMEDIUM
• High FOV
• low FOV X _MagnificationLOW_
• MagnificationHIGH
• REMEMBERwe only use a ruler for LOW FOV

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Lets Practice

• If the low FOV 2mm, low magnification is 4x,
  medium magnification is 10x and high
  magnification is 40x, calculate the high FOV.

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Lets Practice

• If the low FOV 2mm, low magnification is 4x,
  medium magnification is 10x and high
  magnification is 40x, calculate the high FOV.
• High FOV 2mm x _____4x___
• 40x
• 2 mm x 0.1
• 0.2 mm or 200µm

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Lets Practice

• If the low FOV 2mm, low magnification is 4x,
  medium magnification is 10x and high
  magnification is 40x, calculate the high FOV.
• High FOV 2mm x _____4x___
• 40x
• 2 mm x 0.1
• 0.2 mm or 200µm

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Specimen Size

• You can estimate specimen size using the field of
  view. For example, if your field of view is
  200 µm, and the specimen takes up
  about a quarter of
  the space, you can
  estimate it to be about 50
  µm.

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Drawing Magnification/Scale

• When you are asked to draw what you see under the
  microscope, your drawings will be much larger
  than your specimen. You need to indicate
  approximately how much larger than life your
  drawings are.
• Magnification drawing size or M D
  Actual size A

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Drawing Magnification/Scale

• e.g. a student, observing an amoeba under a
  magnification of 400X, calculates that it is
  about 50?m long. If she then draws the amoeba 8
  cm long, what is the magnification and scale of
  her drawing?
• Magnification drawing size 80000 µm
  Actual size 50 µm
• 1600 X, and the scale is 1cm 6.25 µm.