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GAS CHROMATOGRAPHY

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GAS CHROMATOGRAPHY Mr. Maywan Hariono One of chromatography technique that use a gas as a carrier (mobile phase) The separation is based on the volatility of sample ... – PowerPoint PPT presentation

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Title: GAS CHROMATOGRAPHY


1
GAS CHROMATOGRAPHY
  • Mr. Maywan Hariono

2
  • One of chromatography technique that use a gas as
    a carrier (mobile phase)
  • The separation is based on the volatility of
    sample
  • Same with HPLC, GC is one of advance
    chromatography method and use Rt as a separation
    parameter

3
Phase??
  • Stationary phase octadecyl silane, octasilane,
    aminosilane
  • Mobile phase (gas) He, N2, H2, Ar

4
The principle and instrumentation of GC
5
Principles
  • The volatile sample in the liquid matter is
    injected to the column.
  • The temperature of column is setted in which
    sample can be vaporized.
  • The gas is pumped and then it carries the sample
    (gas) through the column.
  • The sample which has higher boiling point/melting
    point will be retained by column, in contrast the
    lower one will be carried through the column
    faster.
  • Means the higher b.p/m.p wil be lower the Rt and
    vice versa

6
Principles
  • The information from GC machine will be
    translated to the graph by software

7
Komponen2 GC
  1. syringe
  2. Injection port
  3. column
  4. GC oven
  5. detector
  6. Carrier gas
  7. recorder

8
GC Component
  1. Syringe 5-10 microliter in volume

9
GC Component
  • b. Injection port

10
GC Component
  • c. Column

11
Factors influence the GC separation performance
  • i.Boiling point/melting point
  • ii. Column temperature
  • iii. Column length
  • iv. The speed of gas stream
  • v. Internal diameter of column

12
GC APPLICATIONS
  • 1. Pharmaceutical industry
  • 2. Herbal medicine industry
  • 3. Environmental analysis
  • 4. Cosmetics analysis

13
adavntages
  • Precise and accurate
  • Sensitive

14
Disadvantages GC
  • Time consuming
  • Only volatile sample can be determined using GC
  • iii. The sample is damaged after running, can not
    be recollected.

15
Rujukan
  • 1.David G Watson(2005). Pharmaceutical Analysis.
    Second edition. Pp 236-265
  • 2.http//www.en.wikipedia.org/wiki/Gas-chromatogra
    phy.html
  • 3.http//orgchem.colorado.edu/hndbksupport/GC.html
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