BIOCHEMICAL CHANGES IN GERMINATED WHEAT GRAINS TREATED WITH SINAL HERBICIDE BY Prof. Dr. Ehab Abdel-Razik Kamel Aly

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BIOCHEMICAL CHANGES IN GERMINATED WHEAT GRAINS
TREATED WITH SINAL HERBICIDEBY Prof. Dr. Ehab
Abdel-Razik Kamel Aly
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Aim of Study
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?? ??? ????? ?? ??????? ???? ???? ?????
(Triticum aestivum cv. Sakha 8) ????? ?? ??????
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??????? ?????? ?????? ????? - SINAL ??? ???
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?? ??? ??? ?????? ?? ????? ???? (15 ??) ?? ???
???? ???? 12 ????? ?? ???? ????? 25 ???? ?????
(?????? ????? - Control) (??? ????? ???? ?? ??
???)? ??????? ????? ??????? ???? 30
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????????
0.02 ??????? / ??? 1
0.2 ??????? / ??? 2
2.0 ??????? / ??? 3
20.0 ??????? / ??? (??????? ?????? ???) 4
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??????? - Sinal ?? ??? ?????? ???????
???????? ??????? ??????? ????????? (2,6-dichloro
-m-tolyl) - 5,7dimethoxy (1,2,4) triazo
(1,5-pyrimidine-2-sulfonamid)
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Physiological Results??????? ???????????
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Changes in fresh weight, dry weight and water
content in wheat grains germinated in different
concentrations of Sinal herbicide.
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Changes in total carbohydrate and total protein
contents in wheat grains germinated in different
concentrations of Sinal herbicide.
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Changes in IAA oxidase and Peroxidase activities
in wheat grains germinated in different
concentrations of Sinal herbicide. Values listed
are expressed as activity g 1 fresh weight
hour-1.
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Changes in Catalase and Protease activities in
wheat grains germinated in different
concentrations of Sinal herbicide. Values listed
are expressed as activity g 1 fresh weight
hour-1.
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Changes in a-amylase activities in wheat grains
germinated in different concentrations of Sinal
herbicide. Values listed are expressed as
activity g 1 fresh weight hour-1.
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Changes in ß-amylase activities in wheat grains
germinated in different concentrations of Sinal
herbicide. Values listed are expressed as
activity g 1 fresh weight hour-1.
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Changes in IAA oxidase, Peroxidase, Catalase, ?,
? amylases and protease activities in wheat
grains germinated in different concentrations of
Sinal herbicide. Values listed are expressed as
activity g 1 fresh weight hour-1.
Concentrations mg L-1 IAA oxidase Peroxidase Catalase ?-amylase ?- amylase Protease
Control 8.45 47.52 5357 0.76 120 184.6
0.02 5.94 42.24 7286 0.82 290 297.5
0.2 6.68 47.40 7072 0.72 260 241.7
2.0 10.68 48.48 6002 0.64 190 172.6
20 15.05 52.20 4072 0.54 120 150.7
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  ?????? ????? ?????? ???????? ?????????? 2 ? 20
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  ?????? ?? ?????? (20 ???????/???) ??? ??????
  ?????? ?????????? ??? ????? ??????? 22,5 ????
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M 1 2 3 4 5
Electrophoretic banding profiles of seed proteins
extracted in Tris-HCl buffer resulted from
treating Triticum aestivum grains with different
concentrations (0.02, 0.2, 2.0 20.0 mg L-1) of
Sinal.
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Protein electrophoretic pattern of Triticum
aestivum germinated seeds treated with Sinal and
extracted in Tris-HCl buffer.
Number of bands M.W. (kDa) Control Concentrations (mg L-1) Concentrations (mg L-1) Concentrations (mg L-1) Concentrations (mg L-1)
Number of bands M.W. (kDa) Control 0.02 0.20 2.00 20.0
1 65.0
2 63.5 - - -
3 61.0
4 48.0
5 37.5
6 34.0
7 29.0
8 24.0 - -
9 22.5 - - -
10 17.5
Total Total 8 7 8 9 10
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• Cytological Results
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Mitotic index and percentage of the different
mitotic phases in Triticum aestivum root tips
treated with Sinal.
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Types of mitotic abnormalities observed in
treated root tips of Triticum aestivum with the
herbicide Sinal.
1- Slight sticky prophase. 2- Disturbed prophase
with micronucleus. 3- C-metaphase. 4- Disturbed
metaphase
5- Disturbed metaphase with chromosome breakages.
6- Two lagging chromosomes with diagonal
metaphase. 7- Diagonal metaphase. 8- Diagonal
metaphase with micronucleus.
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9- Two chromosome bridges in anaphase. 10-
Laggard chromosomes in anaphase.11- Chromosome
bridge with micronucleus in anaphase. 12-
Chromatin fragments in anaphase.
13- Diagonal anaphase. 14,15,16- Sticky telophase
with chromatin fragment.
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17- Micronucleus in telophase. 18- Micronucleus
in interphase. 19- Multi-micronuclei in
interphase. 20- Trinucleated cell.
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• Thanks