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PRACTICAS DE LABORATORIO EN REPRODUCCION

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Title: PRACTICAS DE LABORATORIO EN REPRODUCCION


1
PRACTICAS DE LABORATORIO EN REPRODUCCION
  • INVESTIGACION EN REPRODUCCION

2
HISTOLOGIA
  • Gonzales GF, Hartinger S, Flores Y, Zárate K, Yi
    P. Cycloheximide prevents production of
    arresting, a fraction of 30-50 kDa obtained from
    seminiferous tubules conditioned medium An
    evidence that this fraction is produced in vitro.
    Asian Journal of Andrology. 2004 6 359-364.
  • Gonzales GF, Miranda S, Nieto J, Fernandez G,
    Yucra S, Rubio J, Yi P, Gasco M. Red Maca
    (Lepidium meyenii) reduced prostate size in rats.
    Reproductive Biology and Endocrinology. 2005
    3(1)5

3
Izquierda ControlDerecha Arresting
4
Control Paracrino
  • Hedger MP, Khatab S, Gonzales GF, de Kretser DM.
    Acute and short term actions of serotonin
    administration on the pituitary-testicular axis
    in the adult rat. Reproduction, Fertility and
    Development. 19957 1101-1109.

5
ESPERMATOGENESIS
  • Gonzales GF, Gasco M, Córdova A, Chung A, Rubio
    J, Villegas L. Effect of Lepidium meyenii (Maca)
    on spermatogenesis in male rats acutely exposed
    to high altitude (4340 m). Journal of
    Endocrinology 2004, 18087-95

6
TRANSILUMINACION
  • Gonzales GF, Del Valle L. Adult rat seminiferous
    tubules secrete a fraction greater 30 KDa to
    regulate spermatogenesis. Human Reproduction
    199510 1435-1443.
  • Almenara A., Escalante G., Gazzo E., Gonzales GF.
    Transillumination to evaluate spermatogenesis
    Effect of testosterone enanthate in adult male
    rats. Archives of Andrology. 2001 4621-27.
  • Gonzales GF, Ruiz A, Gonzales C, Villegas L,
    Córdova A. Effect of Lepidium meyenii (Maca)
    roots, a Peruvian plant on spermatogenesis of
    male rats. Asian Journal of Andrology. 2001
    3231-233.
  • Gonzales GF, Hartinger S, Flores Y, Zárate K, Yi
    P. Cycloheximide prevents production of
    arresting, a fraction of 30-50 kDa obtained from
    seminiferous tubules conditioned medium An
    evidence that this fraction is produced in vitro.
    Asian Journal of Andrology. 2004 6 359-364.
  • Chung F, Rubio J, Gonzales C, Gasco M, Gonzales
    GF. Dose-response effects of Lepidium meyenii
    (Maca) aqueous extract on testicular function and
    weight of different organs in adult rats. Journal
    of Ethnopharmacology. 2005 98143-147.

7
CONTEO DE ESPERMATIDES EN TESTICULO
  • Gonzales C, Rubio J, Gasco M, Nieto J, Yucra S,
    Gonzales GF. Effect of short-term and long-term
    treatments with three ecotypes of Lepidium
    meyenii (MACA) on spermatogenesis in rats.
    Journal of Ethnopharmacology. 2005. En prensa.

8
CONTEO Y MOVILIDAD DE ESPERMATOZOIDES EN EPIDIDIMO
  • Del Valle LJ, Bustos-Obregón E, Gonzales GF.
    Serum reproductive hormone levels and sperm
    production in male adult rats after treatment
    with arresting, a fraction obtained from
    seminiferous tubules conditioned medium.
    Andrologia 2003 35 351-357.
  • Gasco M, Rubio J, Chung A, Villegas L, Gonzales
    GF. Effect of high altitude exposure on
    spermatogenesis and epididymal sperm count in
    male rats. Andrologia 2003, 35 368-374.
  • Gonzales GF, Rubio J, Chung A, Gasco M, Villegas
    L. Effect of alcoholic extract of Lepidium
    meyenii (Maca) on testicular function of male
    rats. Asian Journal of Andrology 2003 5 349-352
  • Gonzales GF, Gasco M, Córdova A, Chung A, Rubio
    J, Villegas L. Effect of Lepidium meyenii (Maca)
    on spermatogenesis in male rats acutely exposed
    to high altitude (4340 m). Journal of
    Endocrinology 2004, 18087-95

9
Epididymal sperm count (106) in male rats treated
for 7 and 42 days with vehicle, Red Maca, Yellow
Maca or Black Maca. Plt0.05 with respect to the
control group.
10
APOPTOSIS DE CELULAS GERMINALES
11
CONTAMINANTES EN REPRODUCCION
  • Eibensteiner L, Del Carpio-Sanz A, Frumkin H,
    Gonzales C, Gonzales GF. Lead exposure and semen
    quality among traffic Police in Arequipa, Peru.
    International Journal of Occupational and
    Environmental Health 2005, 11 161-166.
  • Bustos-Obregon E, Yucra S, Gonzales GF. Lepidium
    meyenii (Maca) reduces spermatogenic damage
    induced by a single dose of malathion in mice.
    Asian Journal of Andrology. 2005 771-76.

12
INTERCAMBIO DE CROMATIDES HERMANAS
13
ESTRÉS OXIDATIVO Y ANTIOXIDANTES EN
ESPERMATOGENESIS
14
CICLO ESTRAL
15
CICLO OVULATORIO Y MENSTRUAL
  • Escudero F, Gonzales GF, Góñez C. Hormone profile
    during the menstrual cycle at high altitude.
    International Journal of Gynecology and
    Obstetrics 199655 49-58
  • Gonzales GF, Villena A. Critical anthropometry
    for menarche. Journal of Pediatric and Adolescent
    Gynecology. 19969139-143

16
Menarquia y Menopausia
  • Gonzales GF, Villena A. Body Mass Index and Age
    at menarche in Peruvian children living at high
    altitude and at sea level. Human Biology 1996
    68 265-275.
  • Gonzales GF, Villena A, Ubilluz M. Age at
    menarche in Peruvian girls at sea level and at
    high altitude Effect of ethnic background and
    socioeconomic status. American Journal of Human
    Biology 19968 (4) 457-464
  • Gonzales GF, Ortíz I. Age at menarche at sea
    level and at high altitude in Peruvian women from
    different ethnic composition. American Journal
    of Human Biol. 19946 627-640

17
Perimenopausia y Menopausia
  • Gonzales GF, Villena A. Age at menopause in
    Central Andean Peruvian Women. MENOPAUSE, The
    Journal of the North American Menopause Society.
    1997432-38
  • Gonzales GF, Villena A, De La Cruz D. Age at
    natural menopause among women in Lima City, Peru.
    International Journal of Gynecology and
    Obstetric. 199757 69-72.
  • Gonzales GF. High serum follicle stimulating
    hormone (FSH) during perimenopause at high
    altitude. International Journal Gynecology and
    Obstetrics. 2000 68159-161.

18
CONDUCTA SEXUAL EN ANIMALES DE EXPERIMENTACION
  • Gonzales GF, Ortega JG, Salazar M. Effect of
    neonatal administration of an antidopaminergic
    drug (Metoclopramide) on sexual behavior of male
    rats. Archives of Andrology, 2000 45137-142.

19
Deseo sexual en varones
  • Gonzales GF, Córdova A, Vega K, Chung A, Villena
    A, Góñez C, Castillo S. Effect of Lepidium
    meyenii (Maca) on sexual desire and its absent
    relationship with serum testosterone levels in
    adult healthy men. Andrologia 2002 34 367-372.

20
SITIOS DE IMPLANTACION Y SACOS EMBRIONARIOS
  • Ruiz-Luna AC, Salazar S, Aspajo NJ, Rubio J,
    Gasco M, Gonzales GF. Lepidium meyenii (Maca)
    increases litter size in normal adult female
    mice. Reproductive Biology and Endocrinology
    2005 3(1)16

21
INFERTILIDAD Y PROCEDIMIENTO DE PREPARACION DE
ESPERMATOZOIDES
  • Gonzales GF, Pella RE. Swim down A rapid and
    easy method to select motile spermatozoa.
    Archives of Andrology 19933029-34

22
ANALISIS DEL LIQUIDO SEMINAL
  • Gonzales GF. The corrected seminal fructose test.
    Archives of Andrology 199433 17-22
  • Gonzales GF, Villena A. Influence of low
    corrected seminal fructose levels on sperm
    chromatin stability in semen from men attending
    an infertility service. Fertility and Sterility
    199767 763-768.

23
ANALISIS DEL LIQUIDO SEMINAL
  • Gonzales GF, Córdova A, Gonzales C, Chung A, Vega
    K, Villena A. Lepidium meyenii (Maca) increased
    semen parameters in adult men. Asian Journal of
    Andrology. 2001 3 301-303 .

24
BIOQUIMICA SEMINAL
  • Gonzales GF. Response of the epididymis, seminal
    vesicles and prostate to the endogenous androgen
    stimulation in men attending an infertility
    service. Andrologia. 2002 34 308-316
  • Gonzales GF. Function of seminal vesicles and
    their role on male fertility. Asian Journal of
    Andrology. 2001 3(4)251-8.

25
  • Gonzales GF, Villena A. True Corrected seminal
    fructose level A better marker of the function
    of seminal vesicles in male infertility.
    International Journal of Andrology. 2001 24
    255-260
  • Gonzales GF, Salirrosas A, Torres D, Sánchez A,
    Villena AE. Clomiphene citrate in the treatment
    of men with high sperm chromatin stability.
    Fertility and Sterility. 1998 69 1109-1115

26
PROSTATA
  • Gonzales GF, Miranda S, Nieto J, Fernandez G,
    Yucra S, Rubio J, Yi P, Gasco M. Red Maca
    (Lepidium meyenii) reduced prostate size in rats.
    Reproductive Biology and Endocrinology. 2005
    3(1)5

27
Prostatic epithelial height in control rats, rats
treated with Red Maca alone, testosterone
enanthate (TE) alone or TERed Maca. A. Red Maca
was administered for 14 days. B. Red Maca was
administered for 42 days. aPlt0.05 respect to
vehicle bPlt0.05 respect to Red Maca cPlt0.05
respect to TE.
28
Prostatic luminal area in control rats, rats
treated with Red Maca alone, testosterone
enanthate (TE) alone or TERed Maca. B. Red Maca
was administered for 42 days. aPlt0.05 respect to
vehicle bPlt0.05 respect to Red Maca cPlt0.05
respect to TE.
29
Saturación de oxígeno
  • Gonzales GF, Salirrosas A. Pulse oxygen
    saturation and neurologic assessment in human
    neonates after vaginal and cesarean delivery.
    International Journal of Gynecology and
    Obstetrics. 1998 6363-66
  • Gonzales GF, Salirrosas A. Pulse oxygen
    saturation and neurologic assessment in human
    neonates after vaginal and cesarean delivery.
    International Journal of Gynecology and
    Obstetrics. 1998 6363-66
  • Gonzales GF, Villena AE. Low pulse oxygen
    saturation in post-menopausal women at high
    altitude is related to high serum
    testosterone/estradiol ratio. International
    Journal of Gynecology and Obstetrics,
    200071147-154.

30
Mean pulse oxygen saturation in newborns at 40-41
weeks of gestational age at sea level and at high
altitude (4340 m). Data are mean ? SEM. F value
2146.97, Plt0.0001 (Two-way ANOVA test).Plt0.001
with respect to values at sea level.
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