An Investigation of Chiral Recognition in Electrokinetic Chromatography by means of NMR Spectroscopy

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An Investigation of Chiral Recognition in
Electrokinetic Chromatography by means of NMR
Spectroscopy
NMR spectroscopy was used to characterize the
interactions between chiral ß-blocker drugs and
molecular micelles (MM) of varying hydrocarbon
chain length. These MM are used to separate the
optical isomers of drug molecules. Such
separations are important because the optical
isomers of many drugs have different physiologic
properties. The interactions between the drugs
and MM must be understood at the molecular level
in order to design efficient drug separations.
NMR diffusion experiments showed that differences
in the optical isomers free energies of binding
to the polymer, ???G), are positively correlated
with chiral selectivities or ? values from
chromatography. This correlation shows that the
optical isomers separate based upon differences
in their free energies of binding to the MM.
?-blocker MM ?-blockerMM complex
MM
?-blocker