A new cisplatin analog The synthesis, characterization, selective cytotoxicity and DNA binding studi

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A new cis-platin analog? The synthesis,
characterization, selective cytotoxicity and DNA
binding studies of Tetrachloro(1,10-phenanthroline
-5,6-dione)platinum(IV) X-Ray structure analysis
of Dichloro(1,10-phenanthroline-5,6-dione)platinum
(II).

• Presented by Kimberly Anne Wilson Erica Kennedy
• Advisor Dr. Robert M. Granger, II

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X-Ray Stucture of Pt(dione)Cl2

• Bond lengths (Å) and bond angles (deg) for
  Pt(dione)Cl2

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Infrared Spectrum of Pt(dione)Cl4 taken as KBr
pellet
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Ultraviolet-visible spectrum of Pt(dione)Cl4

• (A) Pt(dione)Cl4 in dry acetonitrile.
• (B) Free 1,10-phenanthroline-5,6-dione (dione) in
  dry acetonitrile.

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Absorption Maxima and Molar Absorptivities for
Pt(dione)Cl4 and free 1,10-phenanthroline-5,6-di
one (dione) in Various Solvents

• Pt(dione)Cl4 Free 1,10-phenanthroline-5,6-dione
  

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Cyclic Voltammograms of Pt(dione)Cl4

• Cyclic Voltammograms of a saturated acetonitrile
  solution of Pt(dione)Cl4 with 0.4 M TBAP.
  Initial potential was 0.50 V vs Ag/AgCl. Arrow
  indicates the original direction of the scan.
  (A) Scan rate of 1000 mV/s. (B) Scan rate of 100
  mV/s. (C) Scan rate of 10 mV/s.

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More Cyclic Voltammograms

• Cyclic Voltammograms of a saturated acetonitrile
  solution of Pt(dione)Cl4 with 0.4 M TBAP.
  Initial potential was 0.50 V vs Ag/AgCl. Arrow
  indicates the original direction of the scan.
  (A) Initial potential sweep in the cathodic
  (negative) direction. (B) Initial potential
  sweep in the anodic (positive) direction.

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Cyclic Voltammogram of 1,10-phenanthroline-5,6-dio
ne

• Cyclic Voltammogram of 1,10-phenanthroline-5,6-dio
  ne (dione). Acetonitrile solution of 0.03M dione
  and 0.20 M TBAP.

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Cytotoxicity Studies

• Response of normal and transformed cell lines to
  Pt(dione)Cl4. Panel A shows the percent
  survival of the normal human cell line WI-38 and
  the transformed line G-401. Panel B shows the
  percent survival of the normal cell line Hs578Bst
  and the transformed line Hs578T. In both cases,
  the cells were exposed to 10-5 M Pt(dione)Cl4
  for 48 hours.

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DNA Binding Studies of Pt(dione)Cl4

• A comparison of the electrophoretic migration
  rates of PolyGG/PolyCC (Blue) PolyGC/PolyGC
  (Red) and PolyAT/PolyAT (Beige) Duplex DNA in the
  absence of Pt(dione)Cl4 vs gels containing 10
  mM Pt(dione)Cl4. Data is reported as a
  reduction in the migration rate. Data is an
  average of four replicates and the standard
  deviation is shown in each bar.

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DNA Binding Studies Comparison of Pt(dione)Cl4
to cis-platin

• My last saved draft didnt have figure 9 from the
  paper. It just needs to be inserted here with the
  caption.

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Acknowledgements

• Financial Support from
• The Jeffress Memorial Trust
• The Commonwealth Health Research Board (CHRB)
• Sweet Briar College Faculty Grants
• Sweet Briar College Summer Research Program
• Participating Researchers
• Dr. Rob Granger (SBC) Dr. Jill Granger (SBC)
• Dr. Robin Davies (SBC)
• Student Researchers
• Eric Mowles (VMI) Lindsay Kinyon (SBC)
• Andy Clark (VMI) Germaine Gottsche (SBC)
• Terrie Luong (RMWC) Mike Wilson (VMI)
• Yen Nguyen (SBC) Shelly Kellog (SBC)
• Emma Kate Payne (SBC) Courtney Arnott (SBC)
• Julia Schmitz (SBC) Laurel Speilman (SBC)
• Brieanne Volger (SBC) Ana Ciric (SBC)