Mycobacterium florentinum, a new species isolated from humans

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Mycobacterium florentinum, a new species
isolated from humans

• Enrico Tortoli

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M. simiae-related mycobacteria

• Slow growth
• Short helix 18 in the 16S rDNA
• Known organisms
• until 1993 M. simiae
• 2004 13 species

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The strains

• 1. isolated twice from the sputum of an elderly
  patient with lung cancer
• 2. isolated twice from the sputum of an elderly
  patient with hemoptysis
• 3. isolated from the stools of an AIDS patient
• 4. isolated from the excised lymph node of a
  child with cervical lymphadenitis
• 5. isolated three times from the sputum of an
  elderly patient with pulmonary disease
• 6. isolated four times from the sputum of a
  patient, long hospitalized in intensive care due
  to pneumonia imputed to pneumococcus
• 7. isolated twice, at four year interval, from
  the sputum of an elderly woman with fibrosis and
  bronchiectasis

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The strains

• 1. isolated twice from the sputum of an elderly
  patient with lung cancer
• 2. isolated twice from the sputum of an elderly
  patient with hemoptysis
• 3. isolated from the stools of an AIDS patient
• 4. isolated from the excised lymph node of a
  child with cervical lymphadenitis
• 5. isolated three times from the sputum of an
  elderly patient with pulmonary disease
• 6. isolated four times from the sputum of a
  patient, long hospitalized in intensive care due
  to pneumonia imputed to pneumococcus
• 7. isolated twice, at four year interval, from
  the sputum of an elderly woman with fibrosis and
  bronchiectasis

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Biochemical and cultural features

• Growth at 25-37C in two weeks
• Colonies smooth, creamy, nonchromogenic
• Positive biochemical tests urea hydrolysis,
  tellurite reduction
• Negative biochemical tests niacin, Tween 80
  hydrolysis, 3-d arylsulfatase, ß-glucosidase
• Variable biochemical tests nitrate reduction,
  semiquantitative catalase
• Growth inhibited on Mac Conkey agar, 5 NaCl
  Lowenstein Jensen
• Growth not inhibited on p-nitrobenzoate,
  isoniazid, hydroxylamine and oleate

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GLC pattern
M. lentiflavum
M. triplex
FI-93171
240 gt 260
200 gt 220
180 gt TBSA
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HPLC pattern
M. simiae
FI-93171
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HPLC pattern
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Antimicrobial susceptibility

• Full susceptibility to clarithromycin and
  clofazimine only
• High MICs of quinolones and rifamycins

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Genetic sequencing
 
 
 

• 16S rDNA 8 nucleotide mismatch with M. triplex
• ITS 14 nucleotide mismatch with M. triplex

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Phylogenetic tree
FI-93171
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PRA patterns
BstE III
Hae III
Haa I
1 2 3
1 2 3
1 2 3
1. FI-93171 2. M. lentiflavum 3. M. triplex
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A previous report
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M. florentinum sp. nov.

• Most closely related species
• M. triplex
• M. lentiflavum

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M. florentinum sp. nov.

• Most closely related species
• M. triplex
• M. lentiflavum

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Contributors
Carlo Garzelli Laura Rindi Department of
Experimental Pathology, University of Pisa,
Pisa, Italy Nalin Rastogi K. S. Goh TB and
Mycobacteria Unit, Institut Pasteur of
Guadeloupe, Pointe a Pitre, Guadeloupe,
France Gianna Mazzarelli Alessandro
Mariottini Laboratory of Microbiology, Careggi
Hospital, Florence, Italy Pirjo
Torkko Laboratory of Environmental Microbiology,
National Public Health Institute, Kuopio, Finland
Marija-Leena Katila Department of Clinical
Microbiology, Kuopio University Hospital, Kuopio,
Finland