Natural Pesticides and Repellents from Plant Essential Oils

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Natural Pesticides and Repellents from Plant
Essential Oils

• Marc Dolan, Janet McAllister, Nicholas Panella,
  Gabrielle Dietrich, Joseph Piesman, and Joseph
  Karchesy

The findings and conclusions in this presentation
are those of the authors and do not necessarily
represent the views of the Centers for Disease
Control and Prevention
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Acknowledgements

• CDC, DVBID
• Jason Schmidt, Nord Zeidner, Elizabeth Gabitszch,
  Amy Ullmann, Mary Adams, Mei Purvis
• Oregon State University
• Mohammad Khasawneh, Javier Peralta-Cruz
• TL Shulze PhD, Inc
• Terry Schulze
• Freehold Township
• Bob Jordan

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Natural Products with Biocidal and Repellent
Activity against Arthropods

• Isolated 18 compounds from AYC essential oil.
• 14 eremophilane sesquiterpenes, 4 monoterpenes
• Determined biocidal activity against ticks,
  fleas, mosquitoes using laboratory bioassays

Nootkatone

• Use of Novel Compounds for Pest Control
  Insecticidal and Acaricidal Activity of Essential
  Oil Components from Heartwood of Alaska yellow
  cedar. 2005. Panella et al. J. Med. Entomol.
  42352-358.

Nootkatol
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History of Natural Products at DVBID

• US Patent No. 7,129,271 10/31/06
• Compounds for Pest Control and Methods for
  Their Use.
• US Patent No. 7,230,033
  6/12/07 Pest Control
  Compositions and Methods for Their Use.
• U.S. Patents Pending
• CIP - Compounds For Pest Control And
  Methods For Their Use
• EIR Filed 2007
• Activity of Incense Cedar Oil and its
  Derivatives

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Microapplicator Direct Topical Bioassay
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Microapplicator Direct Topical Bioassay
0.2 ul / tick
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Intrinsic Toxicity of AYC and IC components
compared to representative commercially available
natural compounds
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Mosquito Repellency Bioassay
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Evaluation of Technical Grade Commercial
Compounds Natural Products as Tick Repellents

• Technical Grade
• Deet
• Picaridin
• IR3535
• Lemon Eucalyptus Oil
• Natural Products
• Nootkatone
• 3 bioassays - Vertical, Horizontal, Finger

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Human Finger Tick Repellent Assay All Compounds
are Tech Grade tested at 2.5 AI in ETOH
Avg of ticks repelled over 5 trials/time point
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Spraying Plots using a Backpack Sprayer
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Plant-Derived Acaricide Field Trials
Reduction of I. scapularis
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Future of Natural Product Research at DVBID

• Primary goal for 2008 is determine the Mode of
  Action
• Collaboration with Legacy BioDesign
• Assisting with formulations
• Pesticides
• Repellents
• Clothing
• Novozymes
• Combo of nootkatone/carvacrol/Metarhizium

KD
Residual