Isolation and Purification of Coenzyme Q10 and its dietary effects on reproductive physiology on mic

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Isolation and Purification of Coenzyme Q10 and
its dietary effects on (reproductive) physiology
on mice.

• Presented By
• Afroza Khatun
•                          Animal Reproductive
  Physiology Lab.
•         23 March 2007

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What is Coenzyme Q10?

• Coenzyme Q10 (CoQ 10) or ubiquinone or ubiquinol
  is essentially a vitamin or fat soluble
  vitamin-like substance present in every cell of
  the body and serves as a coenzyme for several of
  the key enzymatic steps in the production of
  energy within the cell. It is a biologically
  active quinone and related in structure to
  vitamin K and E.
• Chemically, CoQ10 is known as
• 2,3-dimethyoxy-5-methyl-6-decaprenyl
• -1,4- benzoquinone,

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Sources of Coenzyme Q10

• foods
• heart, liver and kidney
• beef, soy oil, sardines, mackerel, and peanuts.

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Functions of the body

• provide energy for the bodys cell growth and
  maintenance.
• potent intracellular antioxidant which is
  important in its clinical effects.
• prevents abnormal protein synthesis and thus age
  related degenerative disease
• ability to transfer electrons and therefore act
  as an antioxidant
• It has no toxicity and side effects.

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Beneficial effects

• heart conditions (especially heart failure)
• muscular dystrophies
• Parkinson's disease
• cancer (breast cancer)
• diabetes
• HIV/AIDS
• benefit the body and the Brain.
• Mitochondrial disorders

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Introduction

• Coenzyme Q (CQ)- ubiquinone and lipid-soluble
  compound
• CQ is an essential cofactor
• CQ10 -with mitochondrial disorders
• CQ10 improved heart muscle
• High concentrations (CQ and VE) are found inner
  membranes of mitochondria.

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Objectives

• i) Isolation and purification of Coenzyme Q10 in
  different organ of mice.
• ii) To know the dietary effect of Coenzyme Q10 on
  the different organs and  blood.
• iii) To investigate the effect of Coenzyme Q10 on
  the reproduction on mice.
• iv) Finally, to identify the factors which are
  responsible to increase the Q10 in the body.

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Materials and Method

• Preparation of Diet and animals
• 2 diets
• Diet 1 (Control, 100 IU VE)
• Diet 2 (500 mg CQ10/kg 100 IU VE)
• Animals Male ICR mice (n 32 Male and Female
  8 weeks old), n8 per diet

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Sample preparation

• 1) Blood sample
• i) anesthetized with diethyl ether
• ii) collected by cardiac puncture
• iii) centrifuged at 6000?g at room temperature
  for 15 min
• iv) Analyzed for CoQ10 concentration by HPLC
•  

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Sample preparation

• 2) Tissue sample
• i) collect the liver, testis and ovary
• ii) homogenized with distilled water
• iii) extracted by chemicals
• Iv)analysis for CoQ10 by HPLC.

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• Analysis of data
• Data obtained will be analyzed using ANOVA
  followed by Tukeys multiple comparison test.

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Results and Discussions
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Thank you