Infectious Disease Laboratory Surveillance Network: A Powerful Tool for Investigation of Infectious

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Infectious Disease Laboratory Surveillance
Network A Powerful Tool for Investigation of
Infectious Disease Outbreak

• Xu Jianguo
• State Key Laboratory for Infectious Diseases
  Prevention and Control (China CDC)
• National Institute for Communicable Disease
  Control and Prevention, China CDC

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How to deal with outbreaks

• Prevent additional cases in the current outbreak
• Reassure the public
• Minimize economic loss
• Identify the causing pathogen
• Identify the relationship in isolates
• Identify the scale of the outbreak
• Identify the source and vehicles of pathogens
• Learn what happened, so as to prevent future
  similar outbreaks
• Learn about a new disease
• Learn something new about an old disease

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Current infection disease surveillance system in
China

• Infectious diseases electro-reporting system,
  good
• Laboratory based surveillance system single
  disease
• Data comparable? poor
• Standardized methods ? On the way

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PulseNet China

• September 24, 2004

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PulseNet China

• September 24, 2004
• Central Lab National Institute of Communicable
  Diseases Control and Prevention, China CDC
• Provincial Laboratories

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A typical set of E. coli O157H7 DNA
fingerprints
Fragment Sizes (in kilobases)
1135 Kb
452.7 Kb
216.9 Kb
76.8 Kb
33.3 Kb
DNA fingerprints
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Examples of PulseNet China in outbreak
investigation
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Outbreak caused by E.coli O157H7 in 1999
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Cases of Meningitis in Anhui Province in 2004/05
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Percentage of Type AH1 of Neisseria meningitidis
Serogroup C in China
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Investigation of Cholera outbreak related with
turtles at Sichuan in 2004
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PulseNet, a new concept for infectious diseases
control and prevention

• Identical equipments
• Identical reagents
• Identical protocols
• Identical software for analysis data
• Trained personals
• Remote data analysis
• Data sharing

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New technology

• No experimental variation in results
• Allowing direct inter-laboratory comparisons
• Offer more precise information on strain
  relatedness than does PFGE.
• Declining costs of DNA sequencing technology
• Simplify subtype data production and analysis
• Improve strain discrimination
• Reduce response time for outbreak investigation
  and other public health questions.

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PulseNet International

• Global collaboration to deal infectious diseases
  outbreaks and bio-attacks
• Data sharing among countries

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Thank for you attention