Refined Approach for Diagnosis and Targeted Treatment for Lung Cancer

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Refined Approach for Diagnosis and Targeted
Treatment for Lung Cancer
Analysis of Affinity for Tumor-Specific Binding
Reagents
Patient Biopsy
Targeted Smart Therapeutic
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Immunolocalization of Phage on H2009 cells
Nuclear Stain
Flourescein Channel
Phase Contrast
Control Phage
TP H2009.1 Phage
400 X magnification
Oyama et al (2003) Cancer Lett. 202, 219-230
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Targeting of H2009 cells by H2009 MAP-SAQDot605
Pre-Incubated Random MAP
Pre-Incubated H2009.1MAP
200X Magnification, 5s
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Cell-Staining of H2009 cells with TP
H2009.1MAP-SAQDot605
XY View
YZ View
XZ View
1000X Magnification, Orthogonal Planes
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Structure of Tetrameric Targeting
Peptide-Doxorubicin Conjugate
Doxorubicin
Cell Specific Sequence TP H1299.1
VSQTMRQTAVPLLWFWTGSL TP H2009.1
RGDLATLRQLAQEDGVVGVR B represents a biotinylated
glutamic acid residue
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The TP 2009.1 MAP peptide is able to deliver a
Qdot to a tumor in a mouse model
1. H2009.1-SA-QDot605 IV
2. Circulate peptide conjugate for 24 Hrs
3. Sacrifice animal and assay for
delivery of Qdot to different organs by
microscopy
tumor bearing mouse
200X Magnification
100 mL injection, 31 PeptideQdot SA605, 20 nM
Qdot concentration
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Expression of avß6 in Human Lung Tumors
Adenosquamous Carcinoma
Bronchioloalveolar Carcinoma
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