STERILIZATION AND DISINFECTION

1
STERILIZATION AND DISINFECTION

• CHAPTER 12

2
Terminology

• Disinfection
• Disinfectant
• Antiseptic
• Sterilization

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Chemical Antimicrobials

• Potency
• Phenol coefficient
• Filter paper method
• Use dilution test

4
Disinfectant Selection Criteria

• Time of action
• Safety
• Microbe range

• Penetration
• Preparation
• Cost
• Odor

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Mechanisms of Action

• Protein damage

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Mechanisms of Action

• Membrane damage
• Other

7
Disinfectant Types

• Soaps/detergents
• Acids/Alkalis
• Heavy Metals
• Halogens

8
Disinfectants (cont.)

• Alcohols
• Phenolics
• Oxidizers
• Alkylating agents
• Dyes

9
Physical Antimicrobial Agents

• Heat Application
• Dry Heat
• Moist Heat
• Pasteurization

10
The Autoclave
11
Physical Antimicrobial Methods

• Refrigeration/Freezing
• Dessication
• Freeze-drying

12
Physical Antimicrobial Methods

• Radiation
• Filtration

13
Physical Antimicrobial Methods

• Osmotic Control