Macromolecular Complexes Controlling Cellular Actin Dynamics

1
Macromolecular Complexes Controlling Cellular
Actin Dynamics
www.bio.brandeis.edu/goodelab Goode Lab Office
Hours this week Thursday 11-1 and 3-4 Friday
11-2
2
Macromolecular Complexes Controlling Cellular
Actin Dynamics
MCB and Biochemistry grad students
www.bio.brandeis.edu/goodelab Goode Lab Office
Hours this week Thursday 11-1 and 3-4 Friday
11-2
3
Actin Architecture and Dynamics
4
Biochemistry
Genetics
Cell imaging
Structure
Systems biology
5
One Project in the Lab Formins
Rho
Autoinhibited
6
Rho
Active
Rho
RBD
FH3
FH1
FH2
Autoinhibited
7
Rho
Active
Rho
RBD
FH3
FH1
FH2
Autoinhibited
8
Rho
Active
Rho
RBD
FH3
FH1
FH2
?
Autoinhibited
9
Rho
Rho
RBD
FH3
FH1
FH2
?
Autoinhibited
FH1
FH2
10
Active
Rho
RBD
FH3
FH1
FH2
?
Profilin
4 minutes at 34C
Wild type
bnr1D, bni1 FH2(ts)
11
Defined FH2 structure and actin binding sites
Linker
Grigorieff (Brandeis)
Knob
Lasso/post
Eck (Harvard Medical School)
12
Actin filament assembly by formins
FH2 dimer
Actin
Barbed end
Pointed end
13
Regulation of Full-length Formin Proteins
14
Activated
Auto-inhibited
R
R
15
Activated
Auto-inhibited
R
R
16
Visualization of Dynamics of Single Actin
Filaments (TIRF microscopy and rhodamine-actin)
17
Projects
Protein machines that nucleate actin
assembly Protein machines that dismantle actin
structures Reconstitution of actin assembly in
cell extracts Imaging of actin dynamics in
living cells
18
Macromolecular Complexes Controlling Cellular
Actin Dynamics
www.bio.brandeis.edu/goodelab Goode Lab Office
Hours this week Thursday 11-1 and 3-4 Friday
11-2