Title: Method Development for Russian Nanobiosafety System Vladimir Popov A.N.Bach Institute of Biochemistr
1Method Development for Russian Nanobiosafety
SystemVladimir PopovA.N.Bach Institute of
Biochemistry RAS
2Goals and aims
- Scientific and methodological basis of safety and
conformity systems (??????-??????????? ?
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parameters to be controlled and their detection
ranges (?????????????? ????????? ? ????????? ??
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3Support of nanosafety RD activities
4Nanosafety projects of the Federal Program
Development of Nanoindustry Infrastructure
5Method development projects of the Federal
Program
- Development of methodology and elaboration of
tools for sampling, identification and detecion
of effects of technogenic nanoparticles in living
organisms - Development of methodology and elaboration of
tools for control of nanoparticles localization
in organs and tissues - Development of methodology and elaboration of
tools for evaluation of effects of nanomaterials
on heredity system - Development of methodology and elaboration of
tools for laboratory in vitro testing of
biological activity of nanomaterials - Development of methodology of biotesting and
elaboration of tools for detection of
nanoparticles in the environment for assessment
of their safety, distribution, accumulation and
effect
6Sectoral projects and Integrated project of the
Federal Program
- Development of regulations and tools for control
of nanoparticles at industrial premises - Development of regulations and tools for control
of nanoparticles content in agricultural
production, foodstuffs and packaging - Development of regulations and tools for control
of nanoparticles content in household chemicals,
perfumes and cosmetics - Integrated project
- Creation of the integrated system to provide
safety in the course of RD, manufacturing,
application, circulation and utilization of
nanomaterials in the Russian Federation
7Characteristics of NP important for biological
safety issues
- Size size distribution
- Surface area
- Charge
- Shape
- Chemical composition
- Particle number
- Structure
- Solubility
- Impurities
- Chemistry reactivity
8Detection of NPs choice of method
- Applicability to real multi-component samples
- Sample preparation should not perverse data on
particle size and aggregation state - Relatively simple methods to exclude artifacts
9Particle characterization
10 Special features of the analytical methods for NP
control
11Difficulties in NP assays
- Detection of NPs in biological matrices
- Currently not sufficient
- Reference materials
- There is a lack of availability
- Protocols and standardization
- A large batch to batch and production variability
of commercially available ENPs - General toxicity tests
- Uncertainty on sensitivity of existing (OECD)
tests methods / protocols for the assessment of
ENPs effects - Mutagenicity, carcinogenicity, neurotoxicity and
immunotoxicity - These are of high concern and difficult to asses
in current systems - Environmental fate and effects
- Currently not fully possible to estimate in
practical terms - Metrics (dose response relations)
- Currently there is no available procedure to
correlate chemico-physical properties of ENPs
with observed biological interactions
12NP fate in the environment
13NP in the environment
Exposure predictions
A. Boxall, 2008
- Very low concentrations have to be detected
- Sample preparation technique should preserve
data on the aggregation state
14Criteria for NP selection
Priority candidate materials for nanotoxicology
reference materials
Carbon blackA, B, C
ZnOA, B
AgA
TiO2A, B, C
Polystyrene (fluorescent) B
Carbon nanotubesA, B
Aitken et al., 2008
15NPs OECD priority list
- Titanium oxide
- Aluminium oxide
- Cerium oxide
- Zinc oxide
- Silicon dioxide
- Polysterene
- Dendrimers
- Nanoclays
- Fullerenes
- Single-walled carbon nanotubes (SWCNTs)
- Multi-walled carbon nanotubes (MWCNTs)
- Silver nanoparticles
- Iron nanoparticles
- Carbon black
16Program coordination
- Bank of standard NPs
- Standard calibration method(s)
- Standard protocols
- Scientific Council responsible for method
standardization between Federal Program
participants
17Thank you for attention!
Colloidal gold nanoparticles
Axes 37.27.9 x 29.65.2 nm
Axes 25.75.8 x 21.03.2 nm
Axes 13.31.9 x 11.91.4 nm
Dynamic light scattering
Electron microscopy data
18(No Transcript)
19ENPs fate in the environment
Oberdorster et al., 2005