S-1007 Multistate Research Committee The Science and Engineering for a Biobased Industry and Economy

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S-1007 Multistate Research CommitteeThe Science
and Engineering for a Biobased Industry and
Economy
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Biochemicals Subgroup
Donal Day, dday_at_agctr.lsu.edu Shulin Chen,
chens_at_wsu.edu Mark Eiteman, eiteman_at_engr.uga.e
du Arun Bhunia, bhuniaa_at_foodsci.purdue.edu Mike
Tumbleson, mtumbles_at_uiuc.edu Caye Drapcho,
cdrapch_at_clemson.edu Danielle Carrier,
carrier_at_comp.uark.edu Cady Engler,
c-engler_at_tamu.edu Jin-Woo Kim,
jwkim_at_uark.edu Ruihong Zhang,
rhzhang_at_ucdavis.edu Lorraine Day,
day_at_lsu.edu Mike Zhang, cmzhang_at_vt.edu Lonnie
Ingram, inhram_at_ufl.edu Terry Walker,
walker4_at_clemson.edu John Benemann, JBenemann_at_ao
l.com Any others? Please sign up
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Hydrogen

Hydrogen

Protein
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Biopharmaceutical Mechanisms

• Macro
• Whole animal system
• Whole plant system (glycosylation limitation)
• Whole insect system ( )
• Micro
• Mammalian Cell Culture
• Plant cell culture
• Insect cell culture (baculovirus mechanism)
• Bacterial and fungal culture (E. coli,
  S.cerevisiae, Pichiae)
• Molecular
• Genomics, Proteomics, Metabolomics, Systems
  Biology
• Chaperone mechanisms

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Byproduct Bioconversions to Products of
Increasing Value
Increasing Product Value
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Examples of Research

• University Collaborations

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Research at Clemson University

• Fungal production of essential fatty acids and
  SFE fractionation
• Walker, Hui, Kuan, Heaton, Cantrell
• Algal production of polar lipids and proposed
  hydrogen co-production
• Drapcho, Brune, Walker
• Microalgae Platform
• Brune, Benemann

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Bioconversion and SFE Process
SFE
i. Raw Material(s) ii. Organism Selection iii.
Metabolic Engineering
Microfiltration
Bioreactor
Monitor-Control Station
Partially-Refined Product
LC and Analysis (HPLC or GC)
Purified Product
Figure 1. Typical bioprocess for bioconversion of
raw materials to nutraceutical and specialty
biochemicals. Primary separation procedures
include ultrafiltration (UF), supercritical fluid
extraction (SFE) and liquid chromatography (LC).
Analytical techniques include gas chromatography
(GC) and high pressure liquid chromatography
(HPLC).
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Samples of oil extracted by different extraction
methods compared to commercial sample of refined
rice bran oil
Soxhlet extracted sample (biotreated bran)

• Soxhlet extracted
• sample
• (raw bran)

SFE extracted sample (biotreated bran)
Commercial refined rice ban oil
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Research at Washington State University

• Manure conversion to sugars
• Analysis of manure components cellulose,
  hemicellulose, lignin, sugar, protein, amino
  acids, and metal element,
• Extensive studies were conducted on pretreatment
• single stage acid hydrolysis, two-stage acid
  hydrolysis,
• dilute acid hydrolysis, concentrated acid
  hydrolysis,
• enzyme hydrolysis
• combined acid and enzyme hydrolysis.
• Cull Potato conversion to lactic acid
• Fungal and bacterial fermentation
• Whey/whey permeate conversion to nisin

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Research at Virginia Tech

• Protein separation from feedstocks
• Aqueous two-phase extractions
• Zhang
• Xylitol Bioproduction

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Research at LSU and University of Arkansas

• Low-cost pretreatment of bagasse
• Delignification using singlet oxygen
• Donal Day (LSU Audubon Sugar Institute)
• Characterization and extraction of antioxidants
  in blackstrap molasses
• Julie Carrier (UARK), D. Day

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