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Restriction Enzymes

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Restriction Enzymes. By: Idongesit Bullard, Peter Gajda, and Serge Farinas. Restriction Enzymes. Found in bacteria and archaea, thought to be evolutionary defense ... – PowerPoint PPT presentation

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Title: Restriction Enzymes


1
Restriction Enzymes
  • By Idongesit Bullard, Peter Gajda, and Serge
    Farinas

2
Restriction Enzymes
  • Found in bacteria and archaea, thought to be
    evolutionary defense mechanism
  • Recognize particular nucleotide sequence and cuts
    double stranded DNA at this point
  • Extremely important for molecular biology
    cloning and restriction site mapping

3
Blunt vs Sticky Ends
  • Blunt and sticky ends are produced by the
    restriction enzyme it depends on the enzyme.
  • Blunt ends are produced when the enzyme makes a
    clean cut at the recognition site.
  • --------------

    ACCGATTAGCGAC ACCGATTA
    GCGAC TGGCTAATCGCTG
    TGGCTCCT CGCTG
  • Sticky ends are produced when the enzyme makes a
    jagged cut at the recognition site
  • --------------
    ACCGATTAGCGAC
    ACCGATTA GCGAC
    TGGCTAATCGCTG
    TGGC TAATCGCTC

4
Restriction Mapping
  • A restriction map shows the specific RE
    recognition sites

Fungal Genetics Stock Center (fgsc). Genes from
Neurospora crassa. Retrieved September 3rd, 2008
from www.fgsc.net/plasmid/plasmid.html
5
Restriction Mapping
  • Multiple REs used
  • Each RE cuts at different locations
  • Fragments move through gel according to size
  • Each REs fragments creates signature bands

National Diagnostics. Retrieved Sept 3rd, 2008
from National Diagnostics.com/images
6
Restriction Mapping
  • Since different REs cut at different locations,
  • Can deduce order by comparing fragment sets

7
Restriction Mapping
8
Uses of Restriction Enzymes
  • Creating genome libraries.
  • -Recombinant DNA technology.
  • Cloning DNA molecules.
  • Studying nucleotide sequence.
  • Medicinal purpose
  • -Insulin

9
Work Cited Page.
  • Campbell, N., and Reece, J. (2002). Biology
    Sixth Edition. San Francisco Benjamin Cummings.
  • Nelson, L., and Cox, M. (2004). Lehringer
    Principles of Biochemistry Fourth Edition. New
    York W.H. Freeman and Company.
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