Fluorosurfactants in MEKC, ITP2000, Bratislava

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The Use of Fluorosurfactant FC-129 in Micellar
Electrokinetic Capillary Chromatography

• Ronny de Ridder1,2, Francesco Damin1, Jetse
  Reijenga2 and Marcella Chiari1
• Institute of Biocatalysis and Molecular
  Recognition, C.N.R., Milano, Italy
• Laboratory of Instrumental Analysis, Eindhoven
  University of Technology,The Netherlands

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FC-129
N-ethyl-N-(heptadecafluorooctyl)sulfonylglycine,
K salt CAS 2991-51-7, Aldrich (Steinheim,
Germany)
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CMC determination
CMCFC-129 0.5 mM CMCSDS 8 mM
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Experimental

• Spectra-Phoresis 1000TM
• (Thermo Separation Products, Freemont, CA, USA)
• Fused silica, 50?m ID, 375 ?m OD
• Lt400 mm, Ld320 mm, V20 kV
• UV detection _at_ 200 nm
• Injection 5.17.103 N/m2 pressure, 4 s
• BGE 12.5 mM Sodium tetraborate pH 9.9

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Results EOF and Micelle Mobility
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Results Homologue phenol series
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Results 2-, 3- and 4-ethylphenol
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Results retention factors
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Conclusions

• EOF- Micelle mobility comparable
• systematic decrease in retention factor
• interesting selectivity differences

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