Diapositiva 1

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5th NCEV WORKSHOP Forlí, March 1st, 2007
UNIQUE ABILITY OF A PLASMID ENCODING RESIDUES
311-654 OF HER-2/neu TO ELICIT PROTECTIVE
IMMUNITY AGAINST TRANSPLANTABLE AND AUTOCHTHONOUS
HER-2/neu CARCINOMAS IN MICE Simona Rolla
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AIM OF THE WORK define epitopes of critical
importance in the induction of protective
immunity against HER-2/neu
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BALB/c VACCINATION
Small variation in the length of the protein
influence the accessibility of epitopes to B and
T cell immunity
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BALB-neuT VACCINATION
When TAA plays a causal role in tumor progression
and is expressed on cell membrane, the humoral
response has an important protective role
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ANTIBODIES ELICITED BY EC4-TM VACCINATION MEDIATE
A VERY EFFICIENT REACTION TO rp185neu

• give rise to extensive rp185neu aggregation and
  internalization from the cell membrane

- trigger a more effective ADCC
EC-TM
EC4-TM
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Conformational epitopes recognized by anti-HER-2
antibodies
353-367 ARAITSDNVQEFDGC Linear epitope
recognized by anti-HER-2 antibodies
CONCLUSIONS A short truncated antigen fully
exposing several B-cell epitopes could address
several limitation of anti Her-2/neu vaccination
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