Chapter 9 Column Chromatography

1
Chapter 9Column Chromatography
2
Column Chromatography (CC)

• Column chromatography is a useful method for
  separation and purification of solids and liquid
  samples under 10 grams.
• CC works on the same principle of TLC except that
  the sample solution flows down a column of silica
  gel or alumina filled with solvent.
• A partition coefficient represents the efficiency
  of the column and is determined by the solubility
  of the solute in the two phases.

3
Column Chromatography (CC)

• Only complete Part 1 (microscale) pp. 210-211,
  Ferrocene and Acetylferrocene.
• Packing the Column
• Make sure your column has a polyethylene frit.
• Pour the silica gel (or alumina) into the column
  so it is 1/2 to 2/3 full.
• Pour this silica gel into a 50 mL beaker and add
  8 mL of hexane.
• Clamp the column in a vertical position.
• Add 2 mL of hexane to the column.
• Stir the hexane silica gel mixture while pouring
  into the column.
• Open the valve and drain the solvent. Do not let
  the solvent level get below the silica gel.
• Repeat the last three steps until all of the
  silica gel is in the column.
• Close the valve with some solvent remaining at
  the top of the column.

4
Column Chromatography (CC)

• Follow the handout for further experimental steps
  and obtain the following
• Weight of crude ferrocene and of crude
  acetylferrocene.
• Melting points of ferrocene and of
  acetylferrocene.
• Percent Recovery

5
Comments

• Acetylferrocene is toxic. Handle with care.
• Hexane is flammable. Be aware of your
  surroundings.
• It should be easy to follow this separation
  process as both compounds are colored.