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Capillary Electrokinetic Separations Lecture Date: April 26th, 2007 Capillary Electrokinetic Separations What is Capillary Electrophoresis? The Basis of ...
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Develop with formaldehyde and carbonate followed by stopping with Glacial acetic ... require a stop solution including 10% Acetic acid. 2D Gel Electrophoresis ...
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Many diverse types of pathogens can contaminate water, ... Excessive microbial growth in water and biofilms that cause appearance, taste and odor problems: ...
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Types of Liquid Chromatography. I. Ion Exchange Chromatography. A. Factors influencing retention ... Chiral 3.5. Hydrophobic 1.1. Supercritical Fluids ...
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Electrophoresis Principle: The movement of charged molecules in an electric field The rate of migration is dependent on the net electrical charge of the molecule ...
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Explain the role of these antigens in tissue engraftment and rejection. ... Due to new allele discovery, retyping results may differ from typing performed ...
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TB outbreaks and the usefulness of molecular typing. Dr Deborah Modha ... Southern blot hybridisation with IS6110. Pattern visualised. 6. Health Protection Agency ...
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Strain diversity (insertions, deletions & rearrangements) give rise to ... Cell suspension. abs ~1.8. Make agarose plugs. Lyse cells and wash plugs. Practice ...
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Are adsorbed onto platelets (some antigens more readily than ... Erythrocytes will adsorb some Class I antigens viz. Bg blood group system (B7,A28, B57...
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Cations in the buffer adsorb on the walls leading to an electrical double layer. ... Tightly adsorbed cations and O- ions on silica surface. Cation rich diffuse layer ...
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Forensic cases -- matching suspect with evidence. Paternity testing ... Missing persons investigations. Mass disasters -- putting pieces back together ...
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Acrylamide in fried or baked goods is produced by the ... Supernatant. Pellet. Resin. Fraction 2. Fraction 1. 216 kDa. 132 kDa. 78 kDa. 45.7 kDa. 32.5 kDa ...
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Pour, spread or spot plate methods. Membrane filter methods ... 'pour plate' or plaque assay for development of phage 'plaques' ...
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These are usually junk DNA. Therefore the variations between ... Primers are designed to ensure that different sites fall in non-overlapping regions. ...
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What are the 2 phases of. B-cell development? Antigen ... Chronic vs acute. Clinical Diagnosis. 1) Microscopy. 2) gel electrophoresis of. serum proteins ...
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DNA consists of two long chains of nucleotides twisted into a double helix and ... Only 4 types in all DNA-Adenine, Cytosine, Guanine, and Thymine ...
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Microarray Basics Part 1: Choosing a platform, setting up, data preprocessing Experimental design What type of microarray What overall design strategy How many ...
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Idiopathic nonmalignant monoclonal. cryoglobulinemia may be related to a unique ... been performed in selected idiopathic cases. Type II Cryoglobulin Disorders ...
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Tandemly repeated DNA (may see in the literature as STRs - Short tandem repeats) ... Varying levels of variation associated with each type. Difficulty in scoring ...
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e = molar absorptivity ( constant for each type of molecule ) ... Also, since molar absorptivity does not change either, it remains the same. for all measurements. ...
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Conceptualise the different techniques based on this interaction ... Deleterious in. Autoimmune diseases. Reumathoid arthritis Lupus. Type 1 diabetes Croh'n disease ...
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Porcine (n=1) 1. Bovine2 (n=9) 3. Deer (n=3) 2. Food3 (n=20) 4 ... type Human Poultry Bovine Porcine Deer Food. I 50 23 4 1 1 18. II 1 0 0 0 0 0. III 1 0 0 0 0 0 ...
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Chapter 5 Molecular Tools for Studying Genes and Gene Activity 5.1 Molecular Separations Gel Electrophoresis Two-dimensional Gel Electrophoresis Ion Exchange ...
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Thin Layer Chromatography (TLC) Paper chromatography, Electrophoresis Anna Drew with grateful acknowledgement for inspirational teaching received at
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Clinical Vignette 6: Insulin. 65 yr old women; 20 yr duration of type 2 diabetes ... Clinical Vignette 7. 19 yr old male; 9 yr duration of type 1 diabetes ...
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Molecular Biology Section G Gene manipulation Content G1 DNA CLONING: AN OVERVIEW G2 PREPARATION OF PLASMID DNA G3 RESTRICTION ENZYMES AND ELECTROPHORESIS G4 LIGATION ...
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Confirm diagnosis to support case management. Identification of subtypes, typing ... 1. Consistence (rice water) 2. Blood. 3. Parasites ...
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Introduction to Separations Science Lecture Date: March 31st, 2008 Introduction to Separations Science What is a Separation? Basic Types of Separations Basic Types of ...
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Forensic BioInformatics (bioforensics.com) ... Forensic DNA Typing' by John M. Butler (Academic Press) Internet ... .com/ (see human identity and forensics) ...
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Ecological Genetics Study of the process of evolution occurring in present-day natural populations Evolution occurs primarily by changing the amount, type, and ...
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DAY 1: Molecular biology, the basics Chapter 1: DNA structure and gene expression, nuclear and organel genomes 53 Chapter 2: DNA replication, PCR, electrophoresis ...
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Salmonella infection is a significant global public health problem. ... Bacteriophage-typing designations of Salmonella Typhimurium. J Hyg 78: 297-300, 1977 ...
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Screening for ISA infection in ISA vaccinated atlantic salmon in the Faroe Islands ... We have identified a full lenght wild type ISAV isolate in Faroe Island ...
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Ecological Genetics. Study of the process of evolution ... Ecological Genetics I ... Population genetics is the study of the amount, type, and distribution of ...
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Nucleic acid database in Japan: DDBJ: http://www.ddbj.nig.ac.jp ... 2D gel electrophoresis: http://biocadmin.otago.ac.nz/fmi/xsl/toothprint/home.xsl ...
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Gel Electrophoresis and Southern Blotting ... A technique called Southern blotting combines gel electrophoresis of DNA ... RFLP analysis by Southern blotting ...
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... (vacuolated and deeply basophilic cytoplasm) Acute Lymphoblastic Leukemia Chronic Lymphocytic Leukemia Body Fluids Types of Body Fluids Cerebrospinal Fluid ...
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FokI - sequence dependent (type IIs r.e.) serial cloning - sequence dependent ... Blue Heron Biotechnology (http://www.blueheronbio.com) DNA 2.0 (http://www.dna20.com ...
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Chapter 4 Proteins as Products The last type of chromatography we are going to discuss is high performance liquid chromatography, or HPLC. The previous methods of ...
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Electrochromatography - A Hybrid Separation Technique Gel Filtration Chromatography + Capillary Electrophoresis = Electrochromatography [info shamelessly taken from ...
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Mystery of the Crooked Cell: Investigate Sickle Cell Anemia Using Gel Electrophoresis Module developed at Boston University School of Medicine Presented by Dr. Dan Murray
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The Chronic Lymphocytic Leukemia (CLL) Prognostic factor Good prognosis Bad prognosis Serum markers: lactate dehydrogenase activity (LDH) 2-mikroglobulin activity ...
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DNA CODIS Combined DNA Index System Database Local, state, national system LDIS, SDIS,NDIS Free set-up, training Legal to collect DNA samples from convicted offenders ...
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Binomial System. Carolus Linneaus. Two-parts latinized, italicized name ... Non-binomial, i.e. Human immunodeficiency virus (HIV) Rotavirus. Viral species ...
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Isolation and Purification of Nucleic Acids: Sample Processing Donna C. Sullivan, PhD Division of Infectious Diseases University of Mississippi Medical Center
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Case study 2: using microsatellites for individual-specific ... Most frequent in human insect plant yeast nematode. GA/CT. Dipterocarp. GA/CT & CA/GT ...
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In the Construct Molecule screen, enter name 'Tutorial1' ... Click the Construct button and select Tutorial as the subset, then click the Overwrite button. ...
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Anneal on opposite strands of the target sequence. ... Tm=2(a t) 4(g c): determine annealing temperature. ... optimize PCR, the annealing temperature and the Mg ...
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