Lentikats Biotechnology in Organic Acid Production

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Lentikats Biotechnology in Organic Acid
Production
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Content

• What is the Lentikats biocatalyst?
• How is the Lentikats biocatalyst produced?
• Benefits of the Lentikats biotechnology
• Lentikats biocatalyst and its usage in organic
  acid production
• Partners
• Guarantees
• Contacts

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What is the Lentikats Biocatalyst?

• Unique shape of the matrix and original
  immobilization procedure enables to encapsulate
  free enzymes or microorganisms (bacteria, yeasts,
  fungi) inside a solid porous carrier from
  polyvinylalkohol (PVA). The advantages of PVA
  cells are
• Excellent physical and mechanical characteristics
  that result in a long-term mechanical stability
• Hardly biodegradable
• No toxic effects
• Lentikats biocatalysts enable easy separation of
  enzymes or microorganisms from the bulk media and
  therefore can be used repeatedly.

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How does the Lentikats Biocatalyst work?

• A maximum surface in combination with the minimum
  volume of Lentikats biocatalyst results in a
  substantial reduction of diffusion limitations
  associated with the transfer of substrate into
  the matrix and the product out of the matrix.

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Why polyvinylalcohol?

• It is an inexpensive material
• Encapsulation takes place under optimal,
  cell-sparing conditions
• It allows a production of hydrogel with excellent
  mechanical properties
• It does not have any side effects on fermentation
• It offers high capacity for the cell loading
• The process is stable and easy to control
• It is a non-toxic material

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How is the Lentikats Biocatalyst produced?
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What can be immobilized?

• Free cells
• Dead cells
• Living non-growing cells
• Living growing cells

• Free or immobilized enzymes
• The size of Lentikats biocatalyst pores varies
  depending on the size and diameter of the
  biocatalyst due to
• type of PVA additives (size of macromolecules)
• concentration of additives
• biomass concentration

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Laboratory Units for the Encapsulation

• Lab scale units with a low production capacity of
  Lentikats biocatalysts (for research purposes)
  are also available

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Lentikats Biocatalyst production line
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What are the benefits of LB?
Biotechnologies that are environmentally
friendly
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and also

• Lower biochemical reaction time
• In comparison with conventional technologies much
  higher yield (i.e. 5 15 higher)
• Reuse of the biocatalyst (sieve separation)
• Reduction of the risk of contamination during
  fermentation processes
• High stability of production processes

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Lentikats Organic Acid Production Solutions

• Lentikats biotechnology is used in the following
  processes
• lactic acid production from glucose, sucrose and
  lactose
• (immobilized bacteria Bacillus coagulans)
• gluconic acid production from sucrose and glucose
• (immobilized filamentous fungi Aspergillus
  niger)
• itaconic acid production from sucrose and glucose
• (immobilized filamentous fungi Aspergillus
  terreus).

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Lentikats Biotechnology

• LENTIKATS BIOTECHNOLOGY FOR LACTIC ACID
  PRODUCTION
• Producer bacteria Bacillus coagulans.
• Substrates glucose, sucrose, lactose.
• Production
• batch, fed batch and continuous lactic
  fermentation is stable for all C-substrates.
• saccharose fermentation solutions with 60 g/l of
  saccharose concentrate (The total fermentation
  process was decresed from 48 hours in the first
  conversion to 12 hours in the tenth
  conversion with a lactic acid yield of 91 to 93
  .)
• glucose fermentation solution with 60 g/l glucose
  concentrate (After 14 hours fermentation broths
  consist of 55.2 g /l of lactic acid and 1.2 g /l
  of glucose in the tenth conversion.)
• fermentation productivity is increased from 13.5
  to 17.0 (g LA.dm-3.h-1).

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Lentikats Biotechnology

• LENTIKATS BIOTECHNOLOGY FOR ORGANIC ACIDS
  PRODUCTION
• Producer
• 1) filamentous fungi Aspergillus terreus for
  itaconic acid production
• 2) filamentous fungi Aspergillus niger for
  gluconic acid production
• Substrates sucrose, glucose
• Production
• extensive production of itaconic acid is not
  stable yet
• first results indicate that if the initial
  concentration of sucrose is 50 g/l then the
  concentration of itaconic acid is 18.5 g/l in the
  batch mode process

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What does Lentikats Biocatalyst bring?
Fermentation processes regulation
Optically clear L-lactic acid (99) manufacturing
Biomass regeneration costs decrease
Robustness
High yield of lactic acid (about 20 )
High specific activity
Operational costs decrease
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Delivering on Lentikats Biotechnology Key
Benefits
Significant improvement of operating economic
results
Low risk of contamination
Notable cost reduction
Increased operational costs
Decreased sales revenue
Decreased fermentation volumes
High yields About 91-93
High Productivity Short fermentation time (10
hours)
Reusable Lentikats Biocatalysts
High concentration of Lentikats Biocatalysts in
fermentors
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Our partners in the field of industrial
applications
geniaLab, BioTechnologie-Produkte und Dienstleistungen GmbhBüchnerstraße 7, 38118 Braunschweig, Germany www.genialab.com
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Lentikats Biotechnology guarantee of your
investments and the investment return

• You can test our biocatalyst easily by yourself
  with your substrate in your own plant or plant of
  your choice to see the results.
• We offer you our assistance and full support.
• We offer a long-term cooperation to the
  engineering companies specialising in the design
  of distilleries and are interested in the
  implementation of technologies requiring minimal
  investment at high profitability.

Biotechnologies are changing the world
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Lentikats Biotechnology Investment and
Investment Return Guarantees

• We guarantee for the following
• delivery of production facilities within 24
  months
• delivery of output parameters
• operational stability of Lentikats biocatalyst
  and their activity and productivity
• purchase price stability within 36 months.

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Our partners in the field of research and
development
Institute of Microbiology, Academy of Science of the Czech Republic,Vídenská 1083, 142 20 Praha 4, Krc www.biomed.cas.cz
STU Bratislava, Faculty of chemical and food technology, Institute of biotechnology and food technology, Radlinského 9, 81237 Bratislava, SK www.stuba.sk
ICT Prague, Department of Fermentation Chemistry and bioengineering, Technická 5, 166 28 Praha 6 www.vscht.cz
University of Alberta, Edmonton Alberta, T6G 2J9 Canada www.ualberta.ca
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Lentikats Biotechnology Applications

• Lentikats Biocatalysts and applied Lentikats
  Biotechnology also delivers additional value and
  benefits in the following sectors
• waste and industrial water treatment,
• distillery industry bioethanol production,
• pharmaceutics production of robust
  biocatalysts,
• food industry whey processing, production of
  special sugars carbohydrates and
  syrups

Biotechnologies are changing the world
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Contacts
Laboratory INBIT LentiKats a.s. Kamenice
34 625 00 Brno Czech Republic Tel. 420 511
205 288 Tel. [phone]

• Company Headquarters
• LentiKats a.s.
• Office Building ARGO
• Evropska 846/176a
• 160 00 Prague 6
• Czech Republic
• Tel. [phone]
• Fax [phone]
• E-mail info_at_lentikats.eu
• Production plant
• LentiKats a.s.
• Pod Vinicí 83
• 471 27 Stráž pod Ralskem
• Czech Republic